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Two kinds of sepharose-bound pronases were successfully prepared. The immobilized pronase, directly coupled to cyanogen-bromide activated sepharose, retains 22.6 of original specific activity against casein. However, ω-aminoalkyl sepharose immobilized pronases, in which extension arms of w-aminoalkyl group (number of -CH₂- is 8, 10, and 12) are used, retain almost 100% of original specific activity. Studies of enzyme stability, pH dependence, temperature dependence, and Km values are presented.
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